Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: Dose-dependent effect of Mn-rods on the cell viability of ( A ) A549 and ( B ) Calu-3 cells after 24 and 48 h incubation at 37 °C. Cell viability was assessed using Alamar Blue metabolic assay and expressed as percentage relative to untreated cells (normalized to 100%). Data are shown as means ± SD. n=3. The statistical analysis was conducted using two-way ANOVA, p<0.05 (*), p<0.01 (**) and p<0.001 (***) compared to negative control (untreated cells) for each time point.

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Incubation, Metabolic Assay, Negative Control

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: ( A ) Cell viability of A549 cells after 24 and 48 h of incubation with Mn-rods (at 10, 20 and 30 µg/mL), assessed by Trypan Blue exclusion assay. Triton X 0.2% used as positive control. Data are shown as means ± SD. n=3. The statistical analysis was conducted using two-way ANOVA, p<0.01 (**) and p<0.001 (***) compared to negative control (untreated A549 cells) for each time point. ( B–F ) correspond to phase-contrast microscopy images of A549 after 48 h of exposure to Mn-rods ( B – D) – A549 cells treated with 10, 20 and 30 µg/mL of Mn-rods, respectively; ( E ) negative control (untreated A549 cells); ( F ) A549 cells incubated with Triton X 0.2%). The scale bar is 100 µm.

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Incubation, Trypan Blue Exclusion Assay, Positive Control, Negative Control, Microscopy

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: TEM images showing the cellular uptake of Mn-rods (10 µg/mL) by A549 and Calu-3 cells. Panels ( A – C ) correspond to A549 cells: ( A and B ) after 24 and 48 h of exposure to Mn-rods, respectively. ( C ) corresponds to untreated A549 cells. Panels ( D – F ) correspond to Calu-3 cells: ( D and E ) show Mn-rods internalization after 24 and 48 h of exposure, respectively. ( F ) corresponds to untreated Calu-3 cells. Yellow dashed circles highlight internalized Mn-rods, and yellow dashed lines mark the areas shown at higher magnification.

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: Relative intracellular ROS levels in A549 cells, measured by DCF fluorescence after 4 h of incubation with Mn-rods (10 µg/mL). Tert-butyl hydroperoxide (TBHP, 250 µM) was used as the positive control. Data are shown as means ± SD. n=3. The statistical analysis was conducted using one-way ANOVA, p<0.001 (***) compared to negative control (untreated A549 cells).

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Fluorescence, Incubation, Positive Control, Negative Control

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: Identification of the highest non-toxic concentration of compounds targeting different signaling pathways in A549 cell line, using the Alamar Blue assay. Cells were incubated with the pan-caspase inhibitor zVAD-fmk, cathepsin B inhibitor CA-074, the receptor-interacting serine/threonine-protein kinase 1 (RIPK1) inhibitor necrostatin-1, the radical trapping antioxidant ferrostatin-1, the lipid reactive oxygen species scavenger liproxstatin-1, or the iron-chelating agent deferoxamine, and with the ferroptosis inducer, RSL-3, for 48 h at 37 °C. Data are shown as means ± SD. n=3. The statistical analysis was conducted using ordinary one-way ANOVA, ns – not significant, p<0.05 (*), p<0.01 (**) and p<0.001 (***). NC- negative control (untreated A549 cells).

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Concentration Assay, Protein-Protein interactions, Alamar Blue Assay, Incubation, Negative Control

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: Effect of RSL-3 (2 µM) and different inhibitors (the radical trapping antioxidant ferrostatin-1, 10 µM, the lipid reactive oxygen species scavenger liproxstatin-1, 10 µM, and the iron-chelating agent deferoxamine, 3 µM, on cell viability of A549 cells, following incubation for 48 h at 37 °C. Alamar Blue assay, data are shown as means ± SD. n=3. The statistical analysis was conducted using ordinary one-way ANOVA, p<0.01 (**) and p<0.001 (***). Negative control- negative control (untreated A549 cells).

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Incubation, Alamar Blue Assay, Negative Control

Journal: International Journal of Nanomedicine

Article Title: Exploring the Potential Role of Manganese-Based Zeolitic Imidazolate Framework Nanoparticles in Cancer Therapy: In vitro Studies Using Lung Cancer Cells

doi: 10.2147/IJN.S578869

Figure Lengend Snippet: Study of the potential mechanisms underlying the effect on the metabolic activity of A549 cells after exposure for 48 h to Mn-rods in the presence or absence of the specified inhibitors (the pan-caspase inhibitor zVAD-fmk (5 µM), cathepsin B inhibitor CA-074 (10 µM), the receptor-interacting serine/threonine-protein kinase 1 (RIPK1) inhibitor necrostatin-1 (20 µM), the radical trapping antioxidant ferrostatin-1 (10 µM), the lipid reactive oxygen species scavenger liproxstatin-1 (10 µM), the iron-chelating agent deferoxamine (3 µM). RSL-3 (2 µM), ferroptosis inducer, was used as a positive control of ferroptosis. Data are shown as means ± SD. n=3. The statistical analysis was conducted using ordinary one-way ANOVA, ns – not significant, p<0.01 (**) and p<0.001 (***). Negative control- negative control (untreated A549 cells).

Article Snippet: Human alveolar epithelial type II cells (A549) and human bronchial epithelial cells (Calu-3) (passages 4–22) from the American Tissue Type Culture Collection (ATCC) were cultured at 37 °C in 5% CO 2 and 95% humidity.

Techniques: Activity Assay, Positive Control, Negative Control